Question

I propose to design a new drug which will act as an inhibitor for an enzyme. If I have used all current information about the mechanism of this enzyme to design this inhibitor and I carefully engineer it with similar chemical properties of the transition state, what type of inhibitor am I attempting to engineer and how will I know if I have succeeded?

Answer 1

A competitive inhibitor, collect kinetic data both in the presence and absence of inhibitor and watch for a change in KM. Hope I’ve helped ;)