Question

What method allows researchers to reduce the expression of a target gene, even in wild type organisms that have not previously been genetically modified?

a. gene knockout
b. TILLING
c. RNAi
d. saturation mutagenesis

Answer 1

RNA interference (RNAi) is the method used to reduce the expression of a target gene in wild type organisms, which works through gene silencing by disrupting the translation of the gene's mRNA.

Step-by-step explanation:

The method that allows researchers to reduce the expression of a target gene, even in wild type organisms that have not previously been genetically modified, is c. RNAi (RNA interference). RNA interference is a gene silencing technology where researchers use small interfering RNAs (siRNAs) or microRNAs (miRNAs) to interfere with the translation of a specific gene's mRNA. This post-transcriptional strategy effectively decreases the level of mRNA transcription and, consequently, the translation of the target protein. This approach doesn't require the organism to have been previously genetically modified, as it can be applied directly to silencing genes in wild type specimens.

Answer 2

c. RNAi

Step-by-step explanation:

RNA interference is technology which uses RNA for increasing or decreasing the expression of the target gene. RNA molecules will act as neutralizing agent by targeting the expression of mRNA and destroys it by preventing its expression in to protein.

. ... RNAi technology is very precise, efficient and much better in its stability than the antisense technology.