Question

A scientist wants to make a DNA fingerprint, and she has used polymerase

chain reaction (PCR) and restriction enzymes. What should her next step be?
O
A. Obtain a sample.
O
B. Use imaging techniques,
O
C. Make a karyotype.
O
D. Run gel electrophoresis.

Answer 1

The next step in DNA fingerprinting after PCR and the use of restriction enzymes is to perform gel electrophoresis to separate the DNA fragments, allowing for the visualization of the unique DNA pattern. Option D

Step-by-step explanation:

After the application of the polymerase chain reaction (PCR) and the use of restriction enzymes, the next step in DNA fingerprinting is to run the DNA fragments through gel electrophoresis.

This technique will separate the DNA fragments based on size by applying an electric current to the gel, where smaller fragments move faster and will be located near the bottom of the gel, while larger fragments move slower and will be positioned closer to the top. The distinct pattern of bands formed as a result of gel electrophoresis can then be visualized and analyzed, providing the unique DNA fingerprint required for various applications. option D

Answer 2

Run gel electrophoresis should be the next step.

Explanation:

In this phase, fragments of the DNA are separated OR segregated based on their sizes. DNA samples are placed in one end of gel and electricity is passed to drag them through the gel. The fragments of DNA are made up of negative charge, that’s why they move across the positive electrode. If this process will continue for long time , DNA will shift off the end of gel. To move through the gel it requires high voltage. If the voltage becomes very high it will lead to melting of gel or smearing of the DNA bands.