Question

This problem has been solved!See the answerYou have a DNA sequence of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with the first enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp respectively. After using the second enzyme, you recover 700-bp and 900-bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which is the correct map?You have a DNA sequence of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with the first enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp respectively. After using the second enzyme, you recover 700-bp and 900-bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which is the correct map?A.) -400/--900-----/300 ---B.) -300-/--400--/--900----C.) -400-/300-/---900-----D.) -400-/-100/--300--/900E.) -900 ------/400--/3---

Answer 1

C.) -400-/300-/---900-----

Step-by-step explanation:

The full DNA fragment has 1600 bp.

-------------------------1600------------------------------

After cutting with the first enzyme two smaller fragments are generated: 400 and 1400 bp.

-------400--------/--------------------1200-------------------

After cutting with the second enzyme two smaller fragments of 700 and 900 bp are generated.

-----------700-------------/----------------900---------------

And after treatment with both enzymes, fragments were 300, 400, and 900 bp:

-------400-------/-300-/----------------900---------------