Question

Assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, and 2600. Give the expected sizes of the restriction fragments following complete digestion. 700, 400, 1400, 2600 300, 700, 2200 300, 700, 1000, 1200 400, 800, 1000 (2 of these) 400, 1200, 1600

Answer 1

700, 300, 1200 and 1000 bp.

Step-by-step explanation:

I'm attaching a figure of the fragments generated after complete digestion of the plasmid with restriction enzymes.

The distance in base pairs from the restriction site at position 400 and 700 is 300, so that is the size of one of the generated fragments.

The next location of a restriction site is 1400, and the distance in bp to the previous site is 700.

Next, there are 1200 bp between the restriction site at locations 2600 and 1400.

Finally, because the plasmid is circular and there is no restriction site at position 0 (or 3200), the final fragment will have a length of:

3200 - 2600 + 400 = 1000 bp.