If substantial L is bound to a large protein, rather than being free in the culture, its ability to enter a cell will be hampered and it is possible that it will not be able to enter at all.
Why would this happen?
- Cell membranes are very selective.
- Large molecules are not easily accepted by membranes.
For a substance to enter a cell, it will need to pass through plasma membranes, which are extremely selective in allowing this entry to occur.
When the substance is linked to a large protein, it becomes a molecule that is too large to pass through the membrane receptors and too complex to pass through the selectivity of the membranes, which opt for simpler molecules.