Question

In order to create the transgenic cow, your lab first needs to create a DNA vector containing the insulin gene. This step involves a considerable amount of scientific terminology. Make sure you understand the meaning of key terms. Match the following terms with their correct definitions.

Answer 1

An exon is a region of DNA that is not transcribed.

A restriction site is the location in the plasmid that is recognized by the restriction enzyme Xhol.

A Ligase is an enzyme that joins DNA fragments together.

Origin of replication is the location on the plasmid where DNA replication begins.

A promoter is a region of DNA that initiates transcription of a gene.

Xhol is a restriction enzyme that looks for the sequence TCGA.

The Anti-biotic resistance gene is a gene that enables you to identify bacterial cells that have taken up the plasmid.