Answer:
See the answer below, please.
Step-by-step explanation:
The restriction fragment length polymorphism (RFLP) corresponds to a technique designed in the 1980s that is used to analyze specific nucleotide sequences of DNA, which are recognized and suffer cuts by restriction enzymes. Said sequences have variability among individuals (they are polymorphisms resulting from said variation in the DNA sequence recognized by the restriction enzyme).
The limitations of RFLP are:
-It is an expensive procedure, requiring DNA libraries.
-The characterization is specific to each species under study.
-The data obtained from the analyzes depend on the restriction enzyme chosen for the assay.