Question

Indicate whether or not the following antibody combinations could be used to successfully perform your assay. If a pair can’t be used successfully, indicate why not. (6pts)

• Rabbit anti-HA polyclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

• Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

• Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-mouse polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no

Answer 1

• Yes
• No;
• Yes

Step-by-step explanation:

• Yes: The assay would be successful since antibody (III) is raised against rabbit therefore it would recognize and bind to the epitopes on antibody (II) because it is also a rabbit antibody.
• No: The assay would unsuccessful since antibody (III) is raised against rabbit and would not bind to the epitopes on antibody (II) which is mouse anti-HA.
• Yes: The assay would be successful and the signal would be amplified since the polyclonal mouse antibody (III) would bind all the epitopes of the monoclonal mouse antibody (II).