Answer:
The statement which is correct is
The evolutionary fitness associated with the heritable trait of DDT resistance changed once DDT use became widespread.
Step-by-step explanation:
In the 1930's, the usage if insecticides was minimal. Hence, the genes for DDT resistance were absent or silent in the fruit flies at that time.
In the 1960's, the DDT resistant forms of fruit fly began occurring at this rate because they were better adapted to survive. According to Darwin, the species which are better able to adapt to the environment survive and are able to reproduce. Hence, with the passage of time the resistant forms of fruit fly will keep reproducing and increase in number.