Question

In the scheme of feeding bacteria to worms to induce an RNAi response in worms, explain the purpose of the following components: lac promoter incorporated into the genome of E. coli, T7 gene incorporated into the genome of E. coli, and T7 promoter on the plasmid introduced into E. coli.

Answer 1

For repression of gene the lac parameters must be incorporated into genome of E.coli

Where for the formation T7 gene,T7 gene must be incorporated into genome of E.coli

For attachment of polymerase to transcript RNA from 5 to 3 direction the T7 on the placimid must be introduced into E.coli

Answer 2

lac promoter incorporated into the genome of E. coli, = for the repression of the gene

T7 gene incorporated into the genome of E. coli,+ for the formation of T7 gene

and T7 promoter on the plasmid introduced into E. coli= for the attachment of Polymerase to transcriped RNA from 5 to 3 direction

Step-by-step explanation: