Question

One cycle in a polymerase chain reaction (PCR) involves incubating the sample at three successive temperatures: 94° C., 50° C., and 72° C. What is the goal of the third step (72° C.)?

Answer 1

Initial extension ( elongation)

Step-by-step explanation:

in PCR cycle , there is a third step called as extension (also called as elongation step) which is performed at 72 ° C. In this step taq polymerase will add nucleotides to the primers attached for the extension of template Strand.