Question

If amino acid Lysine with a pI of 9.7 is dissolved in a gel running buffer of pH 6; at this pH, Lysine is a___________ and it will migrate toward____________ during the native agarose gel electrophoresis

Answer 1

1. Cation

2. Cathode

Step-by-step explanation:

The isoelectric point, designated as pI refers to the nature of the ionizing group (R) of amino acids at a specific pH. The pH at which the net electric charge of an amino acid is zero is called isoelectric point. An amino acid is positively charged below its isoelectric point.

According to the give in formation, the pI of amino acid Lysine is 9.7 but the amino acid is dissolved in the gel running buffer of 6. This means that lysine will be positively charged at pH 6 and is a cation and will migrate towards the negatively charged electrode during agarose gel electrophoresis .