Final answer:
In a species with a mature diploid number of 12 chromosomes, there would be 24 chromosomes present in a mitotic cell immediately after sister chromatids separate.
Step-by-step explanation:
The question asks how many chromosomes are present in a mitotic cell immediately after the sister chromatids have separated in a eukaryotic organism. During mitosis, a diploid cell contains two sets of chromosomes, and just after the sister chromatids separate in anaphase, each individual chromatid is considered a separate chromosome. Therefore, for a species where the mature diploid number is 12 chromosomes, there would be 24 chromosomes present in a mitotic cell after separation of sister chromatids because each original chromosome is now divided.