Question

A segment of DNA is 400 base pairs long and contains 2 restriction sites for the restriction enzyme HindIII. This DNA is cut with HindIII and then run on a gel. How many bands would you expect to see after completing DNA gel electrophoresis and staining the gel?

Answer 1

I would expect to see 3 bands after completing DNA gel electriphoresis and staining the gel.

Step-by-step explanation:

The restriction enzyme Hind3 has two Recognization sites in the 400 base pair long DNA molecule.

As a result the restriction enzyme Hind3 with generate 2 cuts in the target 400 base pair long DNA molecule.

The number of 2 cuts in the target DNA molecule will generate 3 DNA fragments.From this point of view it can be stated that 3 bands will form after completing DNA electrophoresis and staining the gel.