Answer:
The correct answer is - 3' to 5' , 5' to 3'.
Step-by-step explanation:
The process of transcription is responsible for the formation of RNA from DNA and RNA polymerase is the enzyme that is responsible for the addition of new nucleotide in synthesizing RNA.
RNA polymerase reads the DNA template in 3' to 5' direction and synthesize the new RNA strand in 5' to 3' direction. The new nucleotide added to 3' hydroxyl group of existing nucleotide chain by phosphodiester bond. In RNA thymine is replaced by uracil.
Therefore RNA polymerase uses the 3' to 5' DNA template to synthesize a 5' to 3' mRNA.