Question

You have added an irreversible inhibitor to a sample of enzyme and substrate. At this point, the reaction has stopped completely. What can you do to regain the activity of the enzyme?

O Removing the irreversible inhibitor should get the reaction working again.
O The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity.
O Adding more substrate will increase the rate of reaction.
O Adding more inhibitor should get the reaction up to speed again.

Answer 1

Answer: The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity.

Step-by-step explanation:

An irreversible inhibitor is the inhibitor which binds with the enzyme so as to restrict the formation of the enzyme substrate complex. It will bind to the enzyme by forming the covalent bond at the active site of the enzyme this leads to the denaturation of the enzyme. The irreversible inhibitor when binds to the enzyme substrate complex makes it a dead end complex.

A new enzyme must be added to reaction so as to regain the active function of the enzyme as the enzyme is in inactive state at this point.