Final answer:
The transport of protons by a proton pump is mediated by a change in the conformation of a transmembrane carrier protein during active transport.
Step-by-step explanation:
The actual transport of protons by the proton pump is mediated by a transmembrane protein which undergoes a change in its conformation. This process involves carrier proteins that facilitate the movement of substances across cell membranes. Carrier proteins undergo allosteric changes, meaning their shape changes when they bind to the substance they are designed to carry. During active transport, which requires energy often in the form of ATP, these proteins shift their conformation to move substances against concentration gradients. In the case of a proton pump, the movement is typically tied to the accumulation of protons against an electrochemical gradient.