Answer:
A. The independent variable is the concentration of nitrate solution used.
Step-by-step explanation:
The experiment wants to determine the effect of nitrates on number of chloroplasts in euglena. In an experiment, independent variable is controlled by the experimenter and its effect is found on the dependent variable. Value of dependent variable is expected to depend on the value of independent variable.
Since here the experimenter has fixed the concentration of nitrate solution in tanks, it is the independent variable. Its effect is being checked on number of chloroplasts in euglena so number of chloroplasts is the dependent variable. Independent variable might have a positive effect, negative effect or no effect on the dependent variable.