Question

You are attempting to determine KMby measuring the reaction velocity at different substrate concentrations, but you do not realize that the substrate tends to precipitate under the experimental conditions you have chosen. How would this affect your measurement of KM?

Answer 1

The experimentally determined Km would be greater than the true Km because the actual substrate concentration is less than expected.

Step-by-step explanation:

The Michaelis constant (KM) is defined as the substrate concentration at which the reaction rate is half of its maximal value (or in other words it defines the substrate concentration at which half of the active sites are occupied).