Question

A principal problem with inserting an unmodified mammalian gene into a plasmid and then getting that gene expressed in bacteria is that _____.

Answer 1

introns will remain inside the bacteria

Step-by-step explanation:

Introns are the remains of an RNA transcript that do not involve in coding and hence they are trimmed off once the protein is translated from the RNA molecule.

Introns are found more commonly in eukaryotic genomes as compared to bacterial and archaeal genes

Group I introns have an ability to invade tRNA, rRNA and protein through its intervening sequences however their insertion into genes is phenotypically neutral.