Question

Sequence the steps to making recombinant DNA from 1 to 5.

20. The plasmid becomes part of a host cell's chromosome.
21. A DNA fragment is inserted into a plasmid.
22. The DNA fragment replicates during cell division.
23. The plasmid enters a host bacterial cell.
24. A host cell produces a protein that it would not have produced naturally.

Answer 1

1. A DNA fragment is inserted into a plasmid.
2. The plasmid enters a host bacterial cell.
3. The DNA fragment replicates during cell division.
4. The plasmid becomes part of a host cell's chromosome.
5. A host cell produces a protein that it would not have produced naturally.

Step-by-step explanation:

1. A DNA fragment is inserted into a plasmid. This involves using restriction enzymes to cut both the plasmid and the DNA fragment, which generally comes from another organism, at specific sequences. These 'sticky ends' of the cut sites then anneal, forming a recombinant molecule.
2. The plasmid enters a host bacterial cell. Through a process called transformation, the recombinant plasmid is introduced into the host cell where it can be incorporated into the cell's genetic material.
3. The host bacterial cell, now containing the recombinant DNA, is selected and cultivated. During cell division, the DNA fragment replicates along with the cell's own DNA.
4. The plasmid becomes part of a host cell's chromosome, allowing the new genetic information to be maintained within the host.
5. Finally, a host cell produces a protein that it would not have produced naturally, which is the result of the expression of the newly inserted DNA fragment.

Answer 2

A DNA fragment is inserted into a plasmid.

The DNA fragment replicates during cell division.

The plasmid enters a host bacterial cell.

The plasmid becomes part of a host cell's chromosome.

A host cell produces a protein that it would not have produced naturally.

Step-by-step explanation:

Recombinant DNA is the DNA molecules formed invitro or lab condition with the help of a plasmid, bacterial cell as host and a desirable gene or DNA fragment by the technique of genetic engineering or recombination. This is also known as molecular cloning.

The correct order of steps in the DNA recombination process are as follows:

The desired DNA fragment is inserted into a vector or plasmid.

The DNA fragment replicates during cell division.

The plasmid enters a cell of host bacterial

The plasmid becomes part of a host cell's chromosome.

A host cell produces a protein that it would not have produced naturally.