To determine the diffusion rate, measure the change in concentration of a substance over time as it moves through a medium.
In an experiment, inject the chemical into the medium and use methods such as spectrophotometry or visual observation to track its spread. Apply Fick's Law of Diffusion, which says that the diffusion rate is inversely related to the distance traveled, surface area, and concentration gradient.
Determine the diffusion rate by measuring the concentration's change over time and using these parameters to compute the substance's passage through the medium.