Question

Identify the true statements about RNA interference. Select all the true statements.

A. miRNA is cleaved by Dicer enzyme complex
B. Duplex RNA (dsRNA) can suppress the expression of a gene.
C. miRNAs are short duplexes approximately 21 nucleotides long
D. miRNAs suppress gene expression by interfering with transcription
E. RNA interference can temporarily suppress the expression of a target genes
(I think A and C and E are true. I doubt that B is right because RNA need to be single strand to be read, not sure about D)

Answer 1

miRNA is cleaved by Dicer, duplex RNA can suppress gene expression, miRNAs are short duplexes, miRNAs do not interfere with transcription, and RNA interference can temporarily suppress gene expression.

Step-by-step explanation:

The true statements about RNA interference are:

A. miRNA is cleaved by Dicer enzyme complex. Dicer is an endonuclease that cleaves miRNA into shorter molecules.

B. Duplex RNA (dsRNA) can suppress the expression of a gene. dsRNA can be recognized by Dicer and processed into short interfering RNAs (siRNAs) that can target and degrade mRNA, leading to gene suppression.

C. miRNAs are short duplexes approximately 21 nucleotides long. miRNAs are synthesized as longer precursor molecules that are processed by Dicer into mature, single-stranded miRNAs.

D. miRNAs do not suppress gene expression by interfering with transcription. Instead, they bind to mRNA and inhibit translation.

E. RNA interference can temporarily suppress the expression of target genes. By degrading targeted mRNA or inhibiting translation, RNA interference can lead to temporary suppression of gene expression.

Answer 2

Remember that:

a. Dicer cleaves pre-miRNA, wich is a dsRNA.

b. dsRNAs are not functional because RNA interference depends on the RNAi ability to pair with parts or the entire mRNA to be silenced.

c. Yes, they are short.

d. They supress gene expression after transcription.

e. Yes because translation of mRNA is neutralized.