Question

A new protein resembling myosin was reported. Unlike myosin, it binds calcium. Its isoelectric point and molecular weight are very similar to those of myosin. Which methods would best separate the new protein from myosin if those two proteins were in the same buffer solution?

Answer 1

affinity chromatography

Step-by-step explanation:

Affinity chromatography is a method of separating similar molecules that are in the same biochemical substance. This method consists of two phases called stationary and mobile. According to the physical and chemical properties of the molecule, it will be retained in the mobile phase and will pass into the stationary phase. Meanwhile, the molecule that does not have the desired chemical and physical characteristics will not be retained in the mobile phase, being discarded from the process.