Question

What is the final concentration of 25 ug / UL if it is diluted in 1: 2 and then in 1:10 in terms of ug / UL and ng / UL? u=micro

Answer 1

The final concentration in terms μg / μL is
`1.25 \mu g/\mu L`.

The final concentration in terms μg / nL is
`1250 ng\mu L`.

Step-by-step explanation:

Initial concentration = 25 μg / μL

diluted concentration of 25 μg / μL by 1:2 = x

Ratio of final concentration to initial concentration = 1:2

`(x)/(25 \mu g/\mu L)=(1)/(2)`

`x=(25 \mu g/\mu L)/(2)=12.5\mu g/\mu L`

Final concentration after dilution of x by 1:10 = y

`(y)/(x)=(1)/(10)`

`y=(12.5\mu g/\mu L)/(10)=1.25 \mu g/\mu L`

1 μg = 1000 ng

`y=1.25 \mu g/\mu L=1.25 * 1000 ng/\mu L=1250 ng/\mu L`