Question

What must eukaryotes do to initiate transcription of a gene?

a) Bind RNA Polymerase to displace histone proteins that binding DNA in the promoter region.
b)Recruit general transcription factors to produce an open complex and then recruit RNA Polymerase.
c)Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.
d)Open the DNA template and then bind RNA Polymerase at the transcriptional initiation site.
e) Bind transcription factors from enhancer sequences to the RNA Polymerase.

Answer 1

Eukaryotes use transcription factors to facilitate transcription initiation by binding to the promoter region and recruiting RNA polymerase.

Step-by-step explanation:

Like prokaryotic cells, the transcription of genes in eukaryotes requires the actions of an RNA polymerase to bind to a sequence upstream of a gene to initiate transcription. However, unlike prokaryotic cells, the eukaryotic RNA polymerase requires other proteins, or transcription factors, to facilitate transcription initiation. Transcription factors are proteins that bind to the promoter sequence and other regulatory sequences to control the transcription of the target gene. RNA polymerase by itself cannot initiate transcription in eukaryotic cells. Transcription factors must bind to the promoter region first and recruit RNA polymerase to the site for transcription to be established.

Answer 2

c)Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.

Step-by-step explanation:

Transcription leads to formation of RNA from DNA. The process is carried out by RNA Polymerase which forms a RNA strand according to the template DNA strand.

It starts by the formation of pre-initiation complex. RNA Polymerase along with transcription factors binds to the promoter region of DNA and forms the pre-initiation complex. Generally the eukaryotic promoters have a sequence called as TATA box where the transcription factors bind first. Eventually RNA polymerase also binds to the region and the DNA strands separate starting the transcription process.