Answer:
DNA helicase separates the two strands of DNA at a replication fork.
Step-by-step explanation:
To allow DNA replication, two DNA strands should be separated to serve as a template strand. DNA helicases are the enzymes that separate the two parental DNA strands in an energy-dependent way. DNA helicases move along the DNA and separate the two strands by breaking the hydrogen bonds between the complementary base pairs of two DNA strands. The energy released from ATP hydrolysis is used to break the hydrogen bonds.