Question

Portable sequencers and other portable, efficient equipment for molecular analysis make genomic analyses easier to do in a wide range of conditions. These types of analyses can provide a great deal of information, from metabolic profiles to genome sizes. How would researchers accomplish this type of work?

Answer 1

This is the answer of the following questions:

1. isolation of genetic material

2. genome sequencing

3. genome assembly

4. genome annotation/determination of open reading frame

5. comparative genomics

Explanation:

1. Isolation of genetic material -

In all living being genetic material is a 'nucleic acid'. Its is a DNA in most of the living organism but, whereas in some living organisms it is RNA. The rDNA technology is isolated the wanted DNA in its pure form.

2. Genome sequencing -

It is also called WGS, in the full genome sequencing, the research worker collect the sample of the DNA and identify the similarities of 3 billion nucleotides that create human genome.

3. Genome assembly -

It is the process of taking a less quantity of DNA sequence in a large amount and store them back to make the representation of real chromosomes by which the DNA originated.

4. Genome annotation/determination of open reading frame -

Obtaining these two, EST or mRNA sequences from random cDNA clones.

Aligning the expressed sequence to locate other than from which they transcribed, e.g., to the location of gene family members.

Increase genome sequencing.

5. Comparative genomics -

It is a biological research field by which the comparison of the genomic features is done from different organisms. It may include the gene order, DNA sequence, regulatory sequences, and gene.