Question

Define limit of resolution: The limit of resolution of a microscope is the shortest distance between four separate points in a microscopes field of view that can still be distinguished as distinct entities. The limit of resolution of a microscope is the minimum distance or angular separation between two point objects which allows them to be resolved according to the Rayleigh criterion. The limit of resolution of a microscope is a measure of how close together two points can be and still be distinguished from one another when viewed through the microscope. The limit of resolution of a microscope is the clearance distance between the closest surface of the cover glass and the objective front lens.

Answer 1

The limit of resolution of a microscope is the minimum distance or angular separation between two point objects which allows them to be resolved according to the Rayleigh

Step-by-step explanation:

The resolution limit for two objects is clearly defined by Rayleig's criterion, which states that two point sources of light are separated for the angle where the minimum of the central maximum of each source matches this can be described by the equation

sin θ = 1.22 λ/ D

Where λ is the wavelength, D the opening of the microscope

In our case the correct answer is:

"The limit of resolution of a microscope is the minimum distance or angular separation between two point objects which allows them to be resolved according to the Rayleigh"