Scientists have engineered bacteria to produce human proteins such as insulin to help treat diabetes Suppose the unmodified eukaryotic gene coding for insulin is inserted directly into a bacterial chromosome. Select the reasons why no expression would be seen in the bacterial cell for the insulin gene.
a. The bacterial RNA polymerase will not bind to the eukaryotic promoter sequence.
b. The gene will induce attenuation in the bacterial cell, which will cause a delay in the transcription and translation of the insulin gene.
c. The bacterial nucleoid does not have the proper proteins to transcribe the eukaryotic gene.
d. Bacteria cannot remove intronic sequence from a gene, so if the gene for insulin were transcribed, it would translate to a nonfunctional protein.