Lets consider the symmetric matrix
, and the two eigenvectors
and
such as:


with
.
The dot product between
and
can be obtained with:

Using the first eigenvector equation we can find:

Lets transpose it


as
is an scalar

Now, as A is symmetric:

so

Lets take the dot product again:

but this is :



But, the parenthesis is equal to

so


Now, subtracting the dot product


As the eigenvalues are distinct,
can't be 1, so

this implies

so the eigenvectors are orthogonal.