Question

An assay protocol requires you to add 12.5 mg of lysozyme to 1.0 L of buffer. You have a lysozyme stock vial labeled 25 mg/mL. How many microliters (UL) of the stock will you need to add to your solution.

Answer 1

500 uL

Step-by-step explanation:

Our stock is 25 mg/mL. This means for every mL (1000 uL) there are 25 mg of lysozyme, and we need 12.5 mg.

So 25/ 1000 uL = 12.5/ X

X= (12.5 mg x 1000 uL)/ 25

X= 500 uL

We need to add to our solution 500 uL of the lysozyme stock.