Answer:
B. Virginia's farmers took over more land to grow tobacco
Step-by-step explanation:
What caused trouble between Virginia's farmers and Native Americans is that Virginia's farmers took over more of the Native Americans land to grow tobacco. This Virginia's farmers are colonist and took over greater amount of land to grow mainly tobacco. The native Americans were not happy with the Virginia farmers due to this. What also caused trouble was due to the fact that this Virginia farmers also wanted to settle down in some of this Native Americans properties.
This causes rift between both countries.