Question

4. You are preparing 250 mL of gel mix for protein electrophoresis, which requires a final concentration of 1.6% SDS. The lab has a 10% stock solution of SDS. How much SDS stock solution (in mL) do you use? Round your answer to the nearest whole number. (Ch. 2.1)

Answer 1

40 mL

Step-by-step explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2

Where C is the concentration, V the volume, 1 is the initial solution and 2 the final solution. So, the final volume will be 250 mL, with 1.6% of concentration:

10xV1 = 1.6x250

V1 = 40 mL

So, it's necessary 40 mL of the SDS stock solution.