Question

A student needed to prepare 500mL of 1X TAE buffer to run a QC gel. The stock solution in the lab is 5X TAE. What volumes of stock TAE and water are needed to prepare the 1X working TAE buffer?

Answer 1

Explanation:

It shall be 100xl times the number of 1x tae

Answer 2

you need 100ml of 5X TAE and 400ml of water.

Explanation:

You need to use a rule of three:

`C_1V_1=C_2V_2`

where:

`\left \{ {{C_1= 5X} \atop {C_2=1X}} \right.`

and

`\left \{ {{V_1 = V_(TAE)} \atop {V_2=500ml}} \right.`

Therefore:

`V_(TAE) = (V_2*C_2)/(C_1)`

`V_(TAE) = 100ml`

Then just rest the TAE volume to the final Volume and you get the amount of water that you need to reduce the concentration.