Question

What might be a use for fluorescence in situ hybridization (FISH)?

a. For identification of a specific gene in a DNA extraction by hybridization to a DNA probe
b. For identification of a specific gene by hybridization to a DNA probe within live cells that have had their DNA denatured by heat.
c. For identification of an mRNA within an RNA extraction by hybridization to a DNA probe.
d. For identification of both mRNA and DNA in cellular extracts using an RNA probe
e. None of the above

Answer 1

B.

Step-by-step explanation:

Flourescent insitu hybridization-This technique permits the direct analysis of sequences of DNA or RNA in tissues

-Through this technique various chromosomal abnormalities can be determined as it focuses on that part of the DNA for which a complementary probe has been used.

-The method involves the denaturation of DNA in a tissue section followed by the application of a labeled probe which is complementary to the sequence of interest.