Question

A genetic engineer needs to use gene therapy to help a person with cystic fibrosis. Arrange the following steps in the order the engineer would use them.

a. Use only the steps you need. Inject the modified CFRT gene into a fertilized egg and implant into a woman who will be the surrogate mother.
b. Combine the cloned CFRT gene with a disarmed respiratory virus.
c. Clone the CFRT gene from someone with cystic fibrosis.
d. Clone the CFRT gene from someone without cystic fibrosis.
e.Modify the CFRT gene by putting on a different promoter
f. Test the patient’s blood cell DNA with PCR to see if they have the CFRT transgene.
g. Have the patient use an inhaler that contains the modified respiratory virus.

Answer 1

d-b-g-f

Step-by-step explanation:

1. Clone the CFRT gene from someone without cystic fibrosis.

This will make millions of copies of the gene (wild type, not being mutated and thus unable of producing the disease).

2. Combine the cloned CFRT gene with a disarmed respiratory virus.

This step will allow the virus to transport the gene of interest.

4. Have the patient use an inhaler that contains the modified respiratory virus.

This step helps the virus to enter and infect the patient's cells and thus allowing the copies of the transgene to be integrated into the patient's genome.

3. Test the patient's blood cell DNA with PCR to see if they have the CFRT transgene.

This will confirm if the transgene has actually been integrated into patient's genome.