Question

Which protein would elute first from a gel filtration column?

protein C, a homodimer with protomer ????r=11300Mr=11300
protein A, with ????r=27000Mr=27000
protein B, with ????r=58400Mr=58400
protein D, with ????r=15600Mr=15600

Answer 1

The correct answer is "protein B, with ????r=58400Mr=58400".

Step-by-step explanation:

A gel filtration column separates different molecules in a mixture according to their molar masses. The gel particles that comprise a gel filtration column are made of Sephadex, a porous material that retains low-molar-mass molecules. Therefore, the proteins that have the higher molecular masses will be the first to elute since they do not interact with the column. In this case, protein B with a molar mass of 58400 will be the first to elute

Answer 2

Protein B with 58400
`M_(r)`

Step-by-step explanation:

`M_(r)` is an abreviation of relative molecular mass and is a measure of weight in macromolecules such as proteins,.

If you compare and order the macromolecules
`M_(r)` you obtain:

`58400 M_(r) >27000M_(r) >15600M_(r) >11300M_(r)`

Remember that the largest protein will emerge first of the gel filtration column, and so on, until the smalest protein have been emerged. The above due to the smallest protein moves a longer distance than the largest protein.

In view of the above the largest protein is protein B with 58400
`M_(r)`