Answer:
a.A student applies crystal violet to a smear, rinses, then observes.
Step-by-step explanation:
A simple stain is a straightforward staining process that involves no more than one stain. The range of options includes Gram safranin, methylene blue, and Gram crystal violet. These stains are implemented to stain the major part of bacteria by giving off a hydroxide ion or taking in a hydrogen ion, leaving itself with a positive charge. As bacteria have a negative charge, these stains stick to the surface of their cells.