Final answer:
When comparing the effectiveness of different methods to inhibit bacterial growth among the given options, option B, closing previously opened containers, should be the least effective at inhibiting bacterial growth.
Step-by-step explanation:
Closing previously opened containers is the least effective method at inhibiting bacterial growth compared to the other options because it does not change conditions that significantly affect bacterial metabolism and survival.
This method only prevents additional bacteria from entering the container and reduces the oxygen inside; it does not actively kill existing bacteria or create an environment that is significantly hostile to bacterial survival. Unlike the other methods, which involve refrigeration, pickling, sugar concentration, and irradiation, simply closing a container does not drastically change the conditions that affect bacterial metabolism and survival.