Question

The concentration of O-Nitro Phenol is proportional to

Select one:
a. the concentration of b-galactosidase
b. the concentration of Luria Broth
c. the concentation of glucose
d. the concentration of lactose
e. the concentration of ONPG
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Answer 1

a. The concentration of β-galactosidase

Step-by-step explanation:

β-Galactosidase is an enzyme that hydrolyzes ß-D-galactosides like lactose to glucose and galactose.

It also cleaves o-nitrophenyl-β-D-galactoside (ONPG) to yield galactose and o-nitrophenol, which is yellow, and can be measured by its absorption at 420 nm.

If the concentration of ONPG is greater than that of β-galactosidase, the concentration of o-nitrophenol produced is proportional to the concentration of the enzyme.