Question

Place the following sequence of events for the reaction catalyzed by fructose-1,6-phosphate aldolase in the correct order:1) deprotonation and cleavage to release GAP and enamine products2) protonation of the enamine3) conversion of the ketone to the imine derived from lysine4) hydrolysis to release DHAP

Answer 1

The fructose-1,6-bisphosphate aldolase refers to an enzyme that catalyzes a reversible reaction, which dissociates the aldol (fructose-1,6-bisphosphate) into the triose phosphates DHAP and G3P. The sequence of events catalyzed by the given enzyme in the correct sequence is:

a) The ketone gets transformed into the imine derived from lysine.

b) Cleavage and deprotonation take place to discharge GAP and enamine components.

c) Enamine gets protonated.

d) The reaction of hydrolysis takes place to discharge DHAP.