Question

A common column material used in size-exclusion chromatography is dextran, a polysaccharide of glucose. Which type of interaction most likely occurs between proteins and the dextran column material?

Answer 1

Hydrogen bonding

Step-by-step explanation:

• A hydrogen bond is a form of the electrostatic force of attraction which takes place between the hydrogen atom and a more electronegative atom such as nitrogen, fluorine, and oxygen.
• Hydrogen bond falls in the category of weak forces.
• In size-exclusion chromatography, the type of interaction that takes place between the proteins and dextran is hydrogen bonding.
• The hydrogen bonding is possible because dextran, being a polysaccharide of glucose has several hydroxyl groups and thus, they get involved in the hydrogen bonding.