Question

A principal problem with inserting an unmodified mammalian gene into a plasmid and then getting that gene expressed in bacteria is that _____.

A) prokaryotes use a different genetic code from that of eukaryotes
B) bacteria translate only mRNAs that have multiple messages
C) bacteria cannot remove eukaryotic introns
D) bacterial RNA polymerase cannot make RNA complementary to mammalian DNA

Answer 1

C) bacteria cannot remove eukaryotic introns

Answer 2

C) bacteria cannot remove eukaryotic introns

Step-by-step explanation:

Because of that, before inserting eukaryotic DNA in prokaryotic cell, it is required to process it.

Cloning of DNA is a process used in molecular biology to replicate DNA fragments of interest. Enzymes required for this process are restriction enzymes which cut both, DNA of interest and bacterial plasmid that is used as vector and DNA ligases which fragment into plasmid. Recombinant plasmid is inserted into bacteria via transformation.