Answer:
1) The gel electrophoresis technique exploits the difference in size and charge of different molecules in a sample. The DNA or protein sample to be separated is loaded on to a porous gel placed in an ionic buffer medium. On application of electric charge, each molecule having different size and charge will move through the gel at different speeds. The porous gel used in this technique acts as a molecular sieve that separates bigger molecules from the smaller ones. Smaller molecules move faster across the gel while the bulkier ones are left behind. The mobility of the particles is also controlled by their individual electric charge. Two oppositely charged electrodes that are part of the system pull molecules of towards them on the basis of their charge.
3) DNA migration in gel electrophoresis. Gel electrophoresis uses electricity to separate fragments of DNA based on their length. ... The negative charge on the sugar-phosphate backbone of DNA polymers cause them to migrate towards the positive electrode when placed in an electrical field.
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