The Plymouth Colony was one of the first settlements in New England, it was founded by Pilgrims. when the Massachusetts Colony was created by the Puritans, the first difference that we see is that the Pilgrims of the Plymouth Colony were less organized than the Massachusetts Puritans and because of that a lot of Pilgrims died. Another difference is that the Plymouth Colony were separatists, they wanted to practice their faith separated from the others, the Puritans differed because they wanted to reform the church and still be a part of it.