what would have happened if we had cut both the jellyfish glo gene and puc18 plasmid with the EcoR1 restriction enzymes

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asked Feb 13, 2020 18.9k views

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what would have happened if we had cut both the jellyfish glo gene and puc18 plasmid with the EcoR1 restriction enzymes

Biology
high-school

Ingyhere asked

by Ingyhere

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They both will give a complimentary sticky end to each other

Cumhur Ata answered Feb 18, 2020

by Cumhur Ata

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A plasmid is cut with the restriction enzyme BamH1 giving fragments of 3000 and 1000 bp as identified by gel electrophoresis and ethidium bromide staining. In a seperate restriction digest the enzyme EcoR1

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