Answer:
Termination
Step-by-step explanation:
The process of transcription involves creating RNA from a DNA template.
In the last stage of transcription, known as termination, the DNA-RNA hybrid separates from the original strand of RNA. Recognizing the point at which no more bases should be added is necessary for terminating transcription. The DNA sequence needed for termination is known as the terminator and consists primarily of the poly U region. At this point, the enzyme stops adding nucleotides to the developing RNA strand and separates from the DNA template.
This process is used by bacteria in two different ways.
1. Initial termination ( Rho - independent )
In this instance, the transcript strand creates a hairpin shape by creating G-C rich inverted repetitions, which permits the creation of a hair-pin structure at this position. However, the transcription bubble is insufficient to support the DNA-RNA hybrid, and the RNA polymerase is then knocked off.
2. Rho — DEPENDENT
Rho is a protein that is involved in this kind of termination process. The RNA-stimulated helicase Rho factor, which disrupts RNA-DNA hybrids, is ATP dependent. At the rut location, this Rho protein binds to RNA ( Rho utilization site ). Rut has to be free of the ribosome in order to bind Rho. When RNA polymerase stops moving at the rut site, Rho protein catches the polymerase and uses helicase activity to separate the DNA-RNA hybrid.