Statement C, that is, he varies the nitrogen and carbon dioxide levels in the air from one trial to the next would represent a scientific mistake in his experiment.
The hypothesis of Malik was regarding the influence of increased oxygen level on the growth of the red-legged grasshoppers. Thus, the areas of his experiment should be the type of grasshopper, the rate of growth of grasshopper, and a high level of oxygen. Therefore, all the given options are relevant to his experiment, except option C.